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  • Title: False-positive Gram-stained smears.
    Author: Hoke CH, Batt JM, Mirrett S, Cox RL, Reller LB.
    Journal: JAMA; 1979 Feb 02; 241(5):478-80. PubMed ID: 83398.
    Abstract:
    The rate per 1,000 smears showing nonviable Gram-negative bacilli (false-positive smears) increased from a baseline of 10.8 to 38.5 following purchase of new culture-collection devices; the rate decreased to 8.0 following replacement of contaminated culture sets. False-positive reports led to changes in therapy for five patients. In addition to being sterile, commercial culture-collection devices should be certified by the manufacturer as being free of stainable microorganisms or as unsuitable for preparation of Gram-stained smears.
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