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  • Title: Kinetics of the "on-off" change in regulatory state of the muscle thin filament.
    Author: Ishii Y, Lehrer SS.
    Journal: Arch Biochem Biophys; 1993 Aug 15; 305(1):193-6. PubMed ID: 8342950.
    Abstract:
    The excimer fluorescence of pyrene iodoacetamide-labeled tropomyosin bound to actin is a probe of the regulatory state change of the muscle thin filament associated with the cooperative binding of myosin subfragment 1 (S1) (Y. Ishii and S. S. Lehrer, Biochemistry 29, 1160, 1990). In this study, the excimer fluorescence was used to monitor the kinetics of the "on-off" state change due to the ATP-induced dissociation of S1 from actin-tropomyosin. A lag in the fluorescence change indicated that the thin filament was maintained in the "on-state" until more than half of the S1s dissociate. The on-off transition rate was slower than the dissociation of the remaining S1s. These delays in relaxation to the "off-state" may explain some observed kinetic and cooperative effects during tension measurements.
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