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  • Title: Cloning and sequencing of the dnaK region of Streptomyces coelicolor A3(2).
    Author: Bucca G, Smith CP, Alberti M, Seidita G, Passantino R, Puglia AM.
    Journal: Gene; 1993 Aug 16; 130(1):141-4. PubMed ID: 8344522.
    Abstract:
    The dnaK homologue of Streptomyces coelicolor A3(2) strain M145 has been cloned and sequenced. Nucleotide sequence analysis of a 2.5-kb region revealed an open reading frame (ORF) encoding a predicted DnaK protein of 618 amino acids (M(r) = 66,274). The dnaK coding sequence displays extreme codon bias and shows a strong preference for CGY and GGY, for Arg and Gly codons, respectively. The predicted DnaK sequence has a high Lys:Arg ratio which is not typical of streptomycete proteins. The region immediately downstream from dnaK contains an ORF for a GrpE-like protein; the predicted start codon of grpE overlaps the last two codons of dnaK, indicating that the two genes are translationally coupled. This organisation differs from that reported for other prokaryotes.
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