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  • Title: Cloning and sequencing of the URA3 gene of Kluyveromyces marxianus CBS 6556.
    Author: Bergkamp RJ, Geerse RH, Verbakel JM, Planta RJ.
    Journal: Yeast; 1993 Jun; 9(6):677-81. PubMed ID: 8346683.
    Abstract:
    The URA3 gene, coding for orotidine-5'-phosphate decarboxylase, from Kluyveromyces marxianus CBS 6556, was isolated from a genomic DNA library. The K. marxianus URA3 gene encodes a protein of 267 amino acids with a calculated molecular weight of 29.3 kDa. Comparison of the K. marxianus protein with the corresponding enzymes of Saccharomyces cerevisiae and Kluyveromyces lactis showed amino acid sequence identities of 81% and 88%, respectively. Using contour-clamped homogenous electric field gel electrophoresis, the genomic copy was found to be located on chromosome VI. We have used the cloned gene for the construction of a K. marxianus leu2 mutant. This mutant contains no heterologous sequences, which is essential to make it acceptable for application in the food industry.
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