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  • Title: Analysis of asparagine-linked oligosaccharides by sequential lectin-affinity chromatography.
    Author: Yamamoto K, Tsuji T, Osawa T.
    Journal: Methods Mol Biol; 1993; 14():17-34. PubMed ID: 8348233.
    Abstract:
    Various immobilized lectins can be successfully used for fractionation and for structural studies of asparagine-linked sugar chains of glycoproteins (see Note 9). This method needs < 10 ng of a radiolabeled oligosaccharide prepared from a glycoprotein by hydrazinolysis or by digestion with endo-beta-N-acetylglucosaminidases. The fractionation and the structural assessment through the use of immobilized lectins make the subsequent structural studies much easier.
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