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  • Title: Rapid primary microwave-glutaraldehyde fixation preserves the plasma membrane and intracellular structures of the protozoan Tritrichomonas foetus.
    Author: Benchimol M, Goncalves NR, de Souza W.
    Journal: Microsc Res Tech; 1993 Jul 01; 25(4):286-90. PubMed ID: 8358078.
    Abstract:
    Tritrichomonas foetus, a pathogenic protozoan, was used as a model to analyse microwave-stimulated fixation as a procedure of preparation of biological samples for electron microscopy of thin sections and freeze-fracture replicas. Good preservation of the protozoan structure was achieved by microwave-stimulated fixation and Epon polymerization. The membrane structure, as visualized in freeze-fracture replicas, was well preserved.
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