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  • Title: Selenium deficiency suppresses the S-glutathiolation of carbonic anhydrase III in rat hepatocytes under oxidative stress.
    Author: Lii CK, Hendrich S.
    Journal: J Nutr; 1993 Sep; 123(9):1480-6. PubMed ID: 8360774.
    Abstract:
    To examine the modification of reactive sulfhydryls of carbonic anhydrase III (CA III), hepatocytes were prepared by collagenase perfusion from Se deficient and Se-adequate male Sprague-Dawley rats. After 24 h in culture, hepatocytes were treated for 15-30 min with one of two oxidative stressors, t-butyl hydroperoxide (t-BuOOH) or menadione. Modification of CA III was measured by isoelectric focusing/immunoblotting. Formation of glutathione disulfide (GSSG) during oxidative stress was markedly less in hepatocytes of Se-deficient rats than in those of Se-adequate rats. During treatment with t-BuOOH, GSSG formation in hepatocytes from Se-adequate rats reached a maximum at 3 min, and then GSSG was gradually reduced to glutathione. After menadione treatment, intracellular GSSG irreversibly increased in hepatocytes of Se-adequate rats but not in those of Se-deficient rats. A modification of CA III that was reversible by dithiothreitol treatment concurred with the formation of GSSG during treatment with either t-BuOOH or menadione. Although modification of CA III occurred in hepatocytes from Se-deficient rats, the extent of modification was significantly less than in Se adequacy, and the modification was less reversible by dithiothreitol than in hepatocytes from Se-adequate rats. Selenium deficiency may be useful in examining the importance of modification of specific proteins subjected to oxidative stress.
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