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  • Title: [Iron deficient erythropoiesis in the young females--analysis by Percoll discontinuous density gradient].
    Author: Makino M, Wakabayashi E, Takenaka S, Kubota K.
    Journal: Rinsho Byori; 1993 Jul; 41(7):789-94. PubMed ID: 8361050.
    Abstract:
    To observe changes in erythropoiesis in iron deficient subjects, we fractioned red cells by discontinuous density gradient using Percoll. Blood samples were taken from 57 young females of 19-21 years old without abnormal history or abnormal biochemical profiles except anemia or iron deficiency. 6 layers gradient were made and 7 fractions (F1 to F7, from the top to the bottom) were obtained. In the lightness fraction (F3 in nonanemic subjects), reticulocytes were concentrated. From patterns of Hemoglobin A1c concentrations of the fractions, it was inferred that red cells with heavier density were older in ages. In cases with iron deficiency or resulting anemia, low density fraction(s) increased in percentage, with relative decrease in that of higher density fractions, as a result of impairment in hemoglobin production. Subjects in whom the lowest density fraction (F3) was less than 5% were regarded to be normal in terms of erythropoiesis, because the fractional pattern was comparable to that of the normal adult males. Among 57 females examined, 24 (52.6%) showed iron deficient erythropoiesis and 6 (10.5%) were iron deficiency anemia (hemoglobin < 120 g/l). The density gradient fractionation of the red cells was proved to be useful in analyzing abnormal erythropoiesis.
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