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  • Title: Stoichiometry of the human lysosomal carboxypeptidase-beta-galactosidase complex.
    Author: Pshezhetsky AV, Potier M.
    Journal: Biochem Biophys Res Commun; 1993 Aug 31; 195(1):354-62. PubMed ID: 8363614.
    Abstract:
    The understanding of the lysosomal beta-galactosidase-carboxypeptidase-neuraminidase multienzymatic complex structure and function requires an efficient system for dissociation and association of its isolated protein components under controlled conditions. In this paper such a system was used to determine the stoichiometry of the two main components of this complex--beta-galactosidase and carboxypeptidase. The complex, after affinity purification from human placenta, was dissociated at pH 7.5 and beta-galactosidase and carboxypeptidase were separated and purified to homogeneity by FPLC anion-exchange chromatography. The 680 kD complex of beta-galactosidase and carboxypeptidase was reconstituted in vitro by mixing the isolated enzymes in a 1:2 molar ratio at pH 7.5 and then progressively acidifying the medium towards the intralysosomal pH value of 4.75. Under the same conditions, beta-galactosidase and carboxypeptidase independently existed as 306 kDa tetramer and 98 kDa dimer, respectively. Reconstitution experiments with various ratios of purified beta-galactosidase and carboxypeptidase allowed us to conclude that the whole complex is made of 4 beta-galactosidase and 8 carboxypeptidase monomers. Cross-linking of the native and reconstituted complexes with dimethylsuberimidate or glutaric dialdehyde suggested that the native and the reconstituted complexes have the same supramolecular structure.
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