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  • Title: Voltage gradient electrophoresis of nucleic acids on agarose gels.
    Author: Barbieri R, Izzo V, Costa MA, Giudice G, Duro G.
    Journal: Anal Biochem; 1993 Jul; 212(1):168-72. PubMed ID: 8368491.
    Abstract:
    A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described.
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