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Pubmed for Handhelds

PUBMED FOR HANDHELDS

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  • Title: Function of gene 49 of bacteriophage T4 III. Isolation of Holliday structures from very fast-sedimenting DNA.
    Author: Flemming M, Deumling B, Kemper B.
    Journal: Virology; 1993 Oct; 196(2):910-3. PubMed ID: 8372457.
    Abstract:
    Branched DNA molecules were identified in ClaI digests of cytosine containing very fast-sedimenting DNA (VFS-DNACYT) which was isolated from Escherichia coli infected with the multiple mutant 49-GT7 of phage T4. In about 10% of randomly picked ClaI fragments branches with three arms (Y-structures) as well as four arms (Holliday structures) were seen in the electron microscope. Branched structures were absent from ClaI digests after treatment with purified endonuclease VII (gp49) in vitro.
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