These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Glomerular endothelial cell proteoglycans--regulation by TGF-beta 1.
    Author: Kasinath BS.
    Journal: Arch Biochem Biophys; 1993 Sep; 305(2):370-7. PubMed ID: 8373175.
    Abstract:
    Glomerular endothelial cells (G-Endo) may participate in the synthesis of glomerular basement membrane. We studied the metabolism of proteoglycans and its regulation by TGF-beta 1 in bovine G-Endo. The synthesis of cell layer and medium-associated [35S]SO4-labeled macromolecules was significantly increased by TGF-beta 1. On ion exchange chromatography, 80-90% of the 35S-labeled macromolecules in the control medium and cell layer were found to be proteoglycans; TGF-beta 1 increased their synthesis by 1.8-fold but did not alter the anionic charge density. On dissociative Sepharose CL-4B chromatography, the medium proteoglycans were distributed into two peaks of Kav 0.22 (M-I) and 0.44 (M-II). Digestion procedures showed that 39% and 57% of peaks M-I and M-II consisted of HSPG, the remainder being CS/DSPG. The HSPG in peak M-II has the same hydrodynamic size as HSPG present in bovine glomerular basement membrane (N. Parthasarathy and R. G. Spiro, 1987, J. Biol. Chem. 259:12749). Cell layer proteoglycans also resolved into two peaks of Kav 0.33 (C-I) and 0.63 (C-II), respectively, on Sepharose CL-4B chromatography; 39 and 45% of these peaks were made of HSPG. TGF-beta 1 increased the synthesis of CS/DSPG in the media by three-fold without affecting the synthesis of HSPG. Our data suggest that G-Endo may participate in synthesis of glomerular matrix HSPG under physiologic conditions; regulation of G-Endo proteoglycans by TGF-beta 1 may be of relevance in glomerular diseases characterized by matrix expansion.
    [Abstract] [Full Text] [Related] [New Search]