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  • Title: Cytoplasmic poly(ADP-ribose)polymerase from mouse plasmacytoma free messenger ribonucleoprotein particles: purification and characterization.
    Author: Jesser M, Chypre C, Hog F, Mandel P.
    Journal: Biochem Biophys Res Commun; 1993 Sep 15; 195(2):558-64. PubMed ID: 8373396.
    Abstract:
    A cytoplasmic poly(ADP-ribose)polymerase (PARP) was purified from mouse plasmacytoma free messenger ribonucleoprotein particles using chromatography on 3-aminobenzamide affigel-10. The purified protein showed one band at 116 kDa on SDS-polyacrylamide gel electrophoresis and shared similar antigenic sites to the nuclear PARP. An apparent Km for NAD of 100.5 +/- 6.3 microM and a Vmax of 174 +/- 40 nmoles of ADP-ribose incorporated/min/mg protein were observed. RNA was detected in the enzyme preparation and the enzymatic activity was not DNA dependent.
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