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  • Title: Automated sample clean-up with solid-phase extraction for the determination of aflatoxins in urine by liquid chromatography.
    Author: Kussak A, Andersson B, Andersson K.
    Journal: J Chromatogr; 1993 Jul 02; 616(2):235-41. PubMed ID: 8376505.
    Abstract:
    An automated extraction and clean-up procedure was developed for the determination of aflatoxins in human urine at the 50 pg/ml level. Aflatoxins B1, B2, G1 and G2 are captured on C2 extraction columns and simultaneously cleaned up with the aid of a robotic system. The processed samples are analysed by reversed-phase high-performance liquid chromatography. Fluorescence detection was enhanced for aflatoxins B1 and G1 using factorial design optimization of the post-column reactor. Silylation of the glass vials used in the robotic system was of the utmost importance. With non-silylated glass vials, up to 75% of the analytes were lost. Average aflatoxin recoveries were B1 95%, B2 90%, G1 93% and G2 89%.
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