These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Characterization of acid-base transport mechanisms in the kidney cell line RCCT-28A.
    Author: Bello-Reuss E.
    Journal: Kidney Int; 1993 Jan; 43(1):173-81. PubMed ID: 8381889.
    Abstract:
    RCCT-28A cells, a continuous cell line of rabbit cortical collecting tubule origin, have been found to exhibit apical peanut-lectin binding, basal band-3 immunostaining and a transepithelial electrical resistance of 246 +/- 37 omega cm2. For the studies reported, confluent monolayers of RCCT-28A cells were grown on permeable wells and incubated in a control solution or in alkaline solutions by lowering PCO2. Equivalent H+ fluxes (JH+) into the apical solution (nmol.min-1.cm2) were measured in the absence of drugs and in the presence of: amiloride (A, 10(-3) M), N-ethylmaleimide (NEM, 5 mM) and omeprazole (OM, 100 microM) in the apical solution. After preincubation in control solutions JH+ was 21 +/- 2 while A had no effect. Addition of NEM diminished JH+ to 12 +/- 2 (P < 0.005), and OM diminished JH+ to 2 +/- 2 (P < 0.001 vs. control). Monolayers incubated at low PCO2 had a basal JH+ of 11 +/- 5. No effect on JH+ could be demonstrated under these conditions by addition of NEM or OM. Removal of K+ from the apical solution diminished apical acidification by 60%. The inhibitor of H+,K(+)-ATPase Schering 28080 (SCH) was tested at different concentrations and an inhibitory effect was demonstrated (JH+ -2 +/- 1 vs. 18 +/- 1, SCH vs. control, respectively). Probenecid and bafilomycin-A also decreased apical acidification and an apical base-equivalent extrusion was apparent under the inhibitors effect. JH+ was abolished by removal of Cl- from the basolateral solution.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]