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  • Title: Expression of the vesicular stomatitis virus nucleocapsid protein gene in Escherichia coli: analysis of its biological activity in vitro.
    Author: Das T, Banerjee AK.
    Journal: Virology; 1993 Mar; 193(1):340-7. PubMed ID: 8382400.
    Abstract:
    The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactopyranoside, the N protein was synthesized, representing 10 to 20% of total cellular protein. The N protein remained in a nonaggregated form in 1 M NaCl solution. The purified N protein was found to be biologically active as demonstrated by its encapsidation of preformed leader RNA as well as its synthesis of genome length RNA in an in vitro transcription-replication reaction. The availability of recombinant N protein in a sufficient amount would facilitate structure and function studies of this important viral protein.
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