These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A double-cos-site vector containing a multiple cloning site flanked by T7 and T3 RNA polymerase promoters.
    Author: Reilly JD, Silva RF.
    Journal: Gene; 1993 Feb 14; 124(1):135-6. PubMed ID: 8382653.
    Abstract:
    A double-cos-site cosmid vector, c2XMCS, contains a multiple cloning site (MCS) with fifteen restriction sites flanked by phage T7 and T3 RNA polymerase promoters. The two cos sites in the cosmid vector enable the construction of cosmid libraries from unfractionated partial digests of genomic DNA. This simple construct combines the efficiency of a double-cos-site cosmid with the versatility provided by the MCS from pBluescript.
    [Abstract] [Full Text] [Related] [New Search]