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  • Title: Rapid preparation of subsarcolemmal and interfibrillar mitochondrial subpopulations from cardiac muscle.
    Author: Chemnitius JM, Manglitz T, Kloeppel M, Doenst T, Schwartz P, Kreuzer H, Zech R.
    Journal: Int J Biochem; 1993 Apr; 25(4):589-96. PubMed ID: 8385638.
    Abstract:
    1. Subsarcolemmal and interfibrillar mitochondria were prepared with complete recovery from rabbit and porcine heart muscle by upward-flotation during 60 sec of Percoll density gradient centrifugation. 2. Mitochondrial subpopulations were identified and characterized according to buoyant density, electron-microscopy, marker enzyme activities and respiratory performance. 3. ADP-induced state 3-respiration related to latent citrate synthase activity as a marker for structurally intact mitochondria was not significantly different in both mitochondrial subtypes.
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