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  • Title: Analysis of the rhesus cytomegalovirus immediate-early gene promoter.
    Author: Alcendor DJ, Barry PA, Pratt-Lowe E, Luciw PA.
    Journal: Virology; 1993 Jun; 194(2):815-21. PubMed ID: 8389084.
    Abstract:
    A cytomegalovirus (CMV) isolate has been recovered from a rhesus macaque (Macaca mulatta) experimentally infected with simian immunodeficiency virus. The full-length immediate-early gene (IE) was cloned from a genomic library of this primary rhesus CMV isolate (RhCMV) using the African green monkey (AGM) CMV IE gene as probe. The promoter region and the first non-coding exon of the RhCMV IE gene have been sequenced. Nucleotide sequence comparisons from positions -733 to +625 (relative to the start site of transcription at +1) reveal 70 and 48% sequence homology with the AGM CMV IE and the human CMV (HCMV) IE genes, respectively. A region that includes the TATA box, the cis repression signal and the first exon in the RhCMV IE gene sequence is 91 and 76% homologous with the AGM CMV and HCMV IE genes, respectively. In permissive MRC-5 cells infected with RhCMV, synthesis of RNA from the IE gene peaks at 4-8 hr post infection. These studies provide a basis for elucidating molecular mechanisms regulating expression of the RhCMV IE gene and viral replication.
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