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  • Title: Application of silver acetate autometallography and gold-silver staining methods for in situ DNA hybridization.
    Author: Hacker GW, Graf AH, Hauser-Kronberger C, Wirnsberger G, Schiechl A, Bernatzky G, Wittauer U, Su HC, Adam H, Thurner J.
    Journal: Chin Med J (Engl); 1993 Feb; 106(2):83-92. PubMed ID: 8389275.
    Abstract:
    In situ hybridization using biotinylated DNA probes has become an important tool in histopathology. It is well known that the sensitivity of the methods used to demonstrate viral DNA in formalin-fixed and paraffin-embedded specimen depends strongly on the detection system used. In the present study, an optimized in situ DNA hybridization protocol was combined with four different approaches of gold-silver staining methods. For silver enhancement, the recently described method of silver acetate autometallography, a technique allowing highly efficient development without the necessity of dark room illumination has been used. The most efficient detection method found in our experiments was the use of gold-adsorbed anti-biotin antibodies with subsequent silver enhancement. This staining procedure can be completed in 5 hours including hybridization and is a highly sensitive alternative to peroxidase and alkaline phosphatase detection systems.
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