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  • Title: Prostaglandins alter the abundance of messenger ribonucleic acid for steroidogenic enzymes in cultured porcine granulosa cells.
    Author: Li XM, Juorio AV, Murphy BD.
    Journal: Biol Reprod; 1993 Jun; 48(6):1360-6. PubMed ID: 8391333.
    Abstract:
    The effects of prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2) on the accumulation of progesterone and mRNA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and cytochrome P450 side-chain cleavage (P450scc) were determined in luteinized granulosa cells. These cells were aspirated from 3-5-mm follicles of prepubertal pigs and cultured 48 h with 10% serum and then 48 h under serum-free conditions. Cells were then incubated with medium (control), ovine LH (20 ng/ml), (bu)2cAMP (0.5 mM) and doses of PGF2 alpha (0.08-2.0 micrograms/ml) or PGE2 (0.1-1.0 micrograms/ml), or combinations of PGF2 alpha (0.1-1.0 micrograms/ml) or PGE2 (0.1-1.0 micrograms/ml) with and without 20 ng/ml LH. Cultures were terminated after 12 h of incubation, and the abundance of mRNA for 3 beta-HSD and P450scc was determined by hybridization with specific cDNA probes. Parallel cultures were terminated at 24 h for analysis of progesterone in the medium by RIA. The results demonstrated that LH and (bu)2cAMP elevated progesterone by 3- to 6-fold and the accumulation of mRNA for 3 beta-HSD by 1.5 to 3.5 or 4-fold and for P450scc by 2-4-fold. PGF2 alpha reduced the basal level of progesterone accumulation, as well as the steady-state concentrations of mRNA for both 3 beta-HSD and P450scc. PGF2 alpha interfered with LH and (bu)2cAMP induction of 3 beta-HSD and P450scc message, and this interference was dependent on the PGF2 alpha dose employed.(ABSTRACT TRUNCATED AT 250 WORDS)
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