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  • Title: Transcription of Drosophila mobile element gypsy (mdg4) in heat-shocked cells.
    Author: Lyubomirskaya NV, Arkhipova IR, Ilyin YuV.
    Journal: FEBS Lett; 1993 Jul 05; 325(3):233-6. PubMed ID: 8391478.
    Abstract:
    Drosophila melanogaster Schneider 2 line cultured cells were subjected to stable transformation by co-transfection with two plasmids, one of which conferred G418 resistance and another which contained the Drosophila retrotransposon, gypsy (mdg4), under the control of the heat-shock protein 70 promoter. Transcription of the introduced constructs, as well as of endogenous gypsy, was examined under the condition of heat shock. Active degradation of pre-existing gypsy transcripts was observed. During recovery, gypsy transcription was restored, but its termination and/or 3'-end processing became aberrant.
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