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  • Title: Purification, crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from Thermus thermophilus.
    Author: Obmolova G, Kuranova I, Teplyakov A.
    Journal: J Mol Biol; 1993 Jul 05; 232(1):312-3. PubMed ID: 8392587.
    Abstract:
    High yields of inorganic pyrophosphatase from Thermus thermophilus HB8 have been purified to homogeneity using anion exchange and hydrophobic chromatography. Crystals suitable for X-ray analysis were obtained by vapour diffusion using ammonium sulphate as precipitant. They belong to the rhombohedral space group R32, with unit cell dimensions a = b = 110.3 A and c = 82.0 A, with one subunit per asymmetric unit. The crystals diffract to 2.0 A resolution.
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