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  • Title: Specific binding of ATP to extracellular sites on Torpedo acetylcholine receptor.
    Author: Carlson BJ, Raftery MA.
    Journal: Biochemistry; 1993 Jul 27; 32(29):7329-33. PubMed ID: 8393340.
    Abstract:
    The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with increasing concentration of 8-azido-ATP and the inhibition of photolabeling by ATP. Protection studies suggest that the binding sites for the photolabel are unique and are not associated with the cholinergic ligand binding sites.
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