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  • Title: [Study of the self-association of deoxytetraribonucleoside triphosphate molecules d(ApGpCpT) in aqueous solution by one-dimensional and two- dimensional (1)H NMR spectroscopy].
    Author: Veselkov AN, Dévis D, Dymant LN, Parkes Kh.
    Journal: Biofizika; 1993; 38(4):627-35. PubMed ID: 8395895.
    Abstract:
    Equilibrium behaviour of self-complimentary deoxytetranucleotides d(ApGpCpT) in D2O solution has been studied by the method of proton magnetic resonance spectroscopy (500 MHz). Complete assignments of the d(ApGpCpT) protons were obtained from both two-dimensional homonuclear COSY- and NOESY-experiments. Concentration and temperature dependences of tetranucleotide proton chemical shifts have been investigated. The equilibrium association constants and thermodynamical parameters of self-association of the molecules have been calculated using theoretical models and regression equations based on experimental results.
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