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  • Title: Direct enantiospecific HPLC bioanalysis of (R,S)-atenolol on a chiral stationary phase.
    Author: Kofahl B, Henke D, Mutschler E.
    Journal: Chirality; 1993; 5(6):479-82. PubMed ID: 8398605.
    Abstract:
    The simultaneous determination of the enantiomers of the beta1-selective adrenergic antagonist atenolol in human plasma and urine is described. After an alkaline pre-extraction atenolol is extracted from biological material at pH 12.3 using dichloromethane/propan-2-ol. The separation of the underivatized enantiomers is achieved by high-performance liquid chromatography on a chiral stationary phase (Chiralcel OD, cellulose tris-3,5-dimethylphenylcarbamate, coated on silica gel) with fluorimetric detection. (--)-)S)-Pindolol is used as an internal standard. The detection limits of 5 ng/ml enantiomer in plasma and 50 ng/ml enantiomer in urine are sufficient for pharmacokinetic studies after therapeutic doses.
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