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Title: Measurement of binding kinetics using the gel electrophoresis mobility shift assay. Author: Gerstle JT, Fried MG. Journal: Electrophoresis; 1993 Aug; 14(8):725-31. PubMed ID: 8404816. Abstract: The gel electrophoresis mobility shift assay is a technique for the qualitative and quantitative analysis of protein-DNA complexes. The ability to resolve reactants, reaction intermediates and products makes this method particularly well-suited for the measurement of the assembly and dissociation rates of protein-nucleic acid complexes. Here we identify conditions that must be met and variations of the technique that are useful for the measurement of reaction rates.[Abstract] [Full Text] [Related] [New Search]