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  • Title: cDNA cloning and expression of cysteine synthase B localized in chloroplasts of Spinacia oleracea.
    Author: Saito K, Tatsuguchi K, Murakoshi I, Hirano H.
    Journal: FEBS Lett; 1993 Jun 21; 324(3):247-52. PubMed ID: 8405359.
    Abstract:
    The cDNA clones for cysteine synthase B, which is localized in chloroplasts of Spinacia oleracea L., were isolated by screening a library with synthetic oligonucleotides encoding a partial peptide sequence of the purified protein. Nucleotide sequence analysis revealed an open reading frame encoding a polypeptide of 383 amino acids containing a putative transit peptide of 52 amino acids. A bacterial expression vector of the cDNA clone could genetically complement an Escherichia coli auxotroph lacking cysteine synthase and could produce the functionally active and immuno-reactive cysteine synthase in E. coli. RNA blot hybridization suggested that the transcripts were primarily accumulated in leaves of spinach.
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