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  • Title: Production of monoclonal antibodies that inhibit ADP-ribosylation of small GTP-binding proteins catalyzed by Clostridium botulinum ADP-ribosyltransferase C3.
    Author: Toratani S, Sekine N, Katada T, Yokosawa H.
    Journal: FEBS Lett; 1993 Jun 21; 324(3):353-7. PubMed ID: 8405381.
    Abstract:
    Four monoclonal antibodies that inhibited ADP-ribosylation of 23 kDa protein(s) of ascidian eggs catalyzed by Clostridium botulinum ADP-ribosyltransferase C3 were produced. They also inhibited C3-catalyzed ADP-ribosylation of the 24 kDa protein of rat liver cytosol. By the immunoprecipitation technique, it was found that they recognized small GTP-binding proteins of ascidian eggs and mammalian brains, but did not interact with the rat brain activator of the ADP-ribosyltransferase reaction. The antibody can also immunoprecipitate recombinant Rho A irrespective as to whether the Rho A is the GDP-bound form or the GTPrS-bound form. Thus the antibodies are novel and useful tools in analyzing the physiological roles of the Rho family of GTP-binding proteins.
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