These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Assessment of ultrarapid and slow freezing procedures for 1-cell and 4-cell mouse embryos.
    Author: Liu J, Van den Abbeel E, Van Steirteghem AC.
    Journal: Hum Reprod; 1993 Jul; 8(7):1115-9. PubMed ID: 8408496.
    Abstract:
    Three cryopreservation procedures were assessed for the freezing of mouse 1-cell and 4-cell embryos: the slow freezing protocols with dimethylsulphoxide (DMSO, A) and propanediol (PROH, B) and the ultrarapid procedure with DMSO (C), which was described by the Monash University group [A. Trounson et al. (1987) Fertil. Steril., 48, 843-850]. The evaluation of the different procedures included survival after freezing and thawing, further development after in-vitro culture to blastocysts and the ability to implant and to form living fetuses after transfer of early blastocysts to pseudopregnant mice. In-vitro development was lower in all frozen embryos than in the unfrozen controls. Procedures A and B induced comparable results and were significantly better than ultrarapid freezing. When unfrozen blastocysts were transferred to pseudopregnant mice, 64% of them implanted in the uterine wall and 59% developed to living fetuses. For zygotes the percentages of implantation sites and living fetuses were 47 and 33% for A, 52 and 44% for B, and 29 and 17% for C, respectively. When 4-cell embryos were cryopreserved, these results were 54 and 46% for A, 60 and 53% for B and 37 and 23% for C, respectively. In the ultrarapid procedure we also looked at the influence of the freezing solution. To set up a control, mouse zygotes and 4-cell embryos were exposed to DMSO as in the ultrarapid procedure except that they were not frozen; the survival and blastocyst formation was not different from controls, but fewer living fetuses were born (31% for zygotes and 41% for 4-cell embryos versus 67% in the controls).(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]