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  • Title: The production of porphyrins from delta-aminolaevulinic acid by Haemophilus parainfluenzae.
    Author: Luppa P, Jacob K, Ehret W.
    Journal: J Med Microbiol; 1993 Oct; 39(4):262-7. PubMed ID: 8411086.
    Abstract:
    Porphyrin production by the haemin-independent Haemophilus parainfluenzae in the diagnostic porphyrin test, which determines the X-factor requirement in Haemophilus spp., was analysed quantitatively by applying modern high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9-8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.
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