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  • Title: Characterization of a Mg-dependent, Na-inositol co-transport process in cardiac sarcolemmal vesicles.
    Author: Rubin LJ, Hale CC.
    Journal: J Mol Cell Cardiol; 1993 Jun; 25(6):721-31. PubMed ID: 8411197.
    Abstract:
    [3H]-myo-Inositol transport into cardiac sarcolemmal vesicles is both a time- and Na(+)-dependent process. Transport was stimulated 3-fold by 1 microM valinomycin suggesting the process is electrogenic. myo-Inositol transport was dependent upon the presence of Mn2+ or Mg2+ but not Ca2+. Kinetic analysis revealed a high affinity transport process that was saturable (Vmax, 650 +/- 71 pmoles myo-inositol/mg protein/min; Km, 37.7 +/- 1.3 microM) and a low affinity process that was unsaturable up to 1.0 mM substrate. Transport was not inhibited by 1.0 mM of either L-glucose, D- or L-galactose. However, D-glucose and L-fucose at 1.0 mM were inhibitory. Higher concentrations (30 mM) of each of the sugars inhibited transport. myo-Inositol transport was also inhibited by the inositol isomers (1.0 mM), D-chiro-inositol, epi-inositol and scyllo-inositol with scyllo-inositol being most effective. Kinetic analysis established scyllo-inositol as a competitive inhibitor of cardiac myo-inositol transport, increasing the Km for substrate three-fold (122 +/- 21 microM). These data indicate that inositol transport across cardiac sarcolemma is a Mg(2+)-dependent Na+ co-transport process that is electrogenic and stereospecific.
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