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  • Title: Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.
    Author: Paton AW, Paton JC, Manning PA.
    Journal: Microb Pathog; 1993 Jul; 15(1):77-82. PubMed ID: 8412629.
    Abstract:
    The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences.
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