These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing.
    Author: Larder BA, Kohli A, Kellam P, Kemp SD, Kronick M, Henfrey RD.
    Journal: Nature; 1993 Oct 14; 365(6447):671-3. PubMed ID: 8413632.
    Abstract:
    A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.
    [Abstract] [Full Text] [Related] [New Search]