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  • Title: [The occurrence of neuraminidase and N-acylneuraminate pyruvate lyase in Haemophilus paragallinarum and Haemophilus paravium n.sp (author's transl)].
    Author: Hinz KH, Müller HE.
    Journal: Zentralbl Bakteriol Orig A; 1977 Feb; 237(1):72-9. PubMed ID: 842179.
    Abstract:
    14 strains of H. paragallinarum, isolated from fowls suffering from infectious coryza or received from other investigators for comparison, and a collective consisting of 9 strains of the new species H. paravium nova species, also isolated from fowls, were shown to possess both enzymes, neuraminidase (EC 3.2.1.18) and N-Acylneuraminate-pyruvate lyase (EC 4.1.3.3). The detection of the enzymes was made initially by paper chromatography using N-acetyl-neuraminyllactose as substrate and secondly by the colorimetric assay of Warren's thiobarbituric acid method using N-acetylneuraminyllactose, colomic acid, Na salt, bovine submaxillary mucin, human serum and furthermore N-acetyl-neuraminate as substrates for neuraminidase and N-acylneuraminate pyruvate lyase respectively. The enzyme activities of the strains of H. paravium are stronger than those of H. paragallinarum.
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