These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Immunocytochemical characterization of p24, a baculovirus capsid-associated protein.
    Author: Wolgamot GM, Gross CH, Russell RL, Rohrmann GF.
    Journal: J Gen Virol; 1993 Jan; 74 ( Pt 1)():103-7. PubMed ID: 8423444.
    Abstract:
    An open reading frame (ORF 1) located upstream of the polyhedron envelope protein gene in the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) genome was cloned in-frame into a trpE bacterial expression vector. The fusion protein produced by this construct was used for the preparation of a monospecific antiserum. Western blot analysis of extracts from OpMNPV-infected Lymantria dispar cells and Autographa californica NPV (AcMNPV)-infected Spodoptera frugiperda cells detected a 24K protein late in infection. This antiserum also reacted with a 24K protein in preparations of budded and polyhedra-derived virus from OpMNPV and AcMNPV. The 24K protein was not N-glycosylated. Immunoelectron microscopy confirmed that the OpMNPV p24 is associated with nucleocapsids of budded and polyhedra-derived virions.
    [Abstract] [Full Text] [Related] [New Search]