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  • Title: Estrogen enhancement of Ca-, Mg-, and Ca-Mg-stimulated adenosine triphosphatase activity in the chick shell gland.
    Author: Qin X, Klandorf H, Porter DW, Holt SB, Martin WG.
    Journal: Gen Comp Endocrinol; 1993 Jan; 89(1):4-10. PubMed ID: 8428648.
    Abstract:
    The effect of 17 beta-estradiol (E2) on Ca-, Mg-, and Ca-Mg-ATPase activity was investigated in the shell gland of 6-week-old chicks. In the first study, each of 42 birds was implanted with three E2 (Compudose-200) pellets. An additional 6 sham-implanted birds served as controls for measurements of body weight and concentrations of E2 and Ca in plasma. The activities of Mg-, Ca-, and Ca-Mg-ATPase peaked coincident with maximum plasma E2 concentrations 8 days after implantation and then progressively declined in concert with the decline in plasma E2. By 29 days after implantation, the ATPase activities were similar to those measured in birds whose E2 pellets had been removed for 11 days. Concentrations of plasma E2 dropped from peak values of 1676 +/- 317 at Day 8 to 611 +/- 180 pg/ml at Day 29. When birds whose E2 pellets had been removed were reimplanted with three pellets per bird, plasma E2 again increased to 1637 +/- 227 pg/ml. ATPase activity in these reimplanted birds also was greater (P < 0.05) than activities measured in E2-removed or E2-maintained birds. In a second study the Ca-ATPase Km and Vmax were determined in E2-implanted chicks (three pellets per bird) and compared to E2-withdrawn chicks. Five days after reimplantation of chicks with E2, there was a significant increase in both Vmax (3.38 +/- 0.21 vs 2.37 +/- 0.28 micrograms Pi/mg protein/min; P < 0.05) and Km (0.31 +/- 0.02 vs 0.25 +/- 0.01 mM Ca; P < .01).(ABSTRACT TRUNCATED AT 250 WORDS)
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