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  • Title: Identification of two new members of the 1-aminocyclopropane-1-carboxylate synthase-encoding multigene family in mung bean.
    Author: Botella JR, Schlagnhaufer CD, Arteca JM, Arteca RN, Phillips AT.
    Journal: Gene; 1993 Jan 30; 123(2):249-53. PubMed ID: 8428666.
    Abstract:
    The key enzyme regulating ethylene biosynthesis in higher plants is 1-aminocyclopropane-1-carboxylate (ACC) synthase. In mung bean (MB), the existence of three genes encoding this enzyme has previously been reported [Botella et al., Plant Mol. Biol. 18 (1992) 793-797], one of which corresponds to a full-length indole-3-acetic acid-inducible cDNA [Botella et al., Plant Mol. Biol. (1992) 425-436]. In this paper we report the cloning of two new genomic sequences coding for ACC synthase in MB (MAC-4 and MAC-5). MAC-4 is 1340 bp long and encodes 388 amino acids (aa) while MAC-5 is 1393 bp long and encodes for 391 aa. Genomic Southern analysis suggests the existence of only one copy of each gene in the genome.
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