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  • Title: SP6 RNA polymerase containing vaccinia virus for rapid expression of cloned genes in tissue culture.
    Author: Usdin TB, Brownstein MJ, Moss B, Isaacs SN.
    Journal: Biotechniques; 1993 Feb; 14(2):222-4. PubMed ID: 8431286.
    Abstract:
    A hybrid transient expression system, in which tissue culture cells are infected with a recombinant vaccinia virus encoding bacteriophage DNA-dependent RNA polymerase and transfected with a plasmid containing a cloned gene behind the bacteriophage promoter, allows rapid high-level expression in nearly 100% of the cells. In order to extend this system to clones from libraries containing SP6 promoters, a new vaccinia virus was constructed encoding bacteriophage SP6 RNA polymerase.
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