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  • Title: Mixed chimerism after sex-mismatched allogeneic BMT: evaluation of two molecular techniques.
    Author: Viard F, Merel P, Bilhou-Nabera C, Marit G, Comeau F, Gharbi MJ, Febrer F, Belloc F, Lacombe F, Broustet A.
    Journal: Bone Marrow Transplant; 1993 Jan; 11(1):27-31. PubMed ID: 8431708.
    Abstract:
    Two different molecular techniques were used to monitor chimerism following 17 non-T cell-depleted BMTs from female donors to male recipients: pHY10, a Y chromosome-specific probe (Southern or slot blots), and a set of primers for Y chromosome sequence-specific amplification by the polymerase chain reaction (PCR). On Southern blots, male DNA was detectable at a level less than 1% of 10 micrograms DNA while cross-reactivity with autosomal sequences was avoided. On slot blots, male DNA was reliably detectable at levels less than 0.5%, even in small sample (0.5 microgram DNA). With the PCR technique, male DNA was detectable at levels of 1:10(6) to 1:10(7) of 0.5 microgram DNA. Slot blot and PCR results were concordant in 19 of 23 samples. Both techniques demonstrated a constant small mixed chimerism during the first year after BMT and in four of nine patients, this chimerism persisted even longer (up to 29 months after BMT).
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