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  • Title: DIF-resistant expression of a prespore-specific gene in Dictyostelium in vitro development.
    Author: Kubohara Y, Okamoto K.
    Journal: Exp Cell Res; 1993 Feb; 204(2):382-4. PubMed ID: 8440335.
    Abstract:
    In submerged culture, the prespore-specific gene, D19, of Dictyostelium discoideum was found to be expressed in the early stages of development, even in the presence of 1 nM of DIF-1 (stalk differentiation inducing factor). This concentration of DIF-1 later caused the degradation of the previously accumulated D19 mRNA concomitant with the induction of the prestalk-specific genes ecmA/ecmB and eventually 85% of cells differentiated into stalk cells. These results suggest that prespore differentiation occurs at least transiently even in the presence of DIF-1.
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