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  • Title: Cross-ligation and exchange reactions catalyzed by hairpin ribozymes.
    Author: Komatsu Y, Koizumi M, Sekiguchi A, Ohtsuka E.
    Journal: Nucleic Acids Res; 1993 Jan 25; 21(2):185-90. PubMed ID: 8441626.
    Abstract:
    The negative strand of the satellite RNA of tobacco ringspot virus (sTobRV(-)) contains a hairpin catalytic domain that shows self-cleavage and self-ligation activities in the presence of magnesium ions. We describe here that the minimal catalytic domain can catalyze a cross-ligation reaction between two kinds of substrates in trans. The cross-ligated product increased when the reaction temperature was decreased during the reaction from 37 degrees C to 4 degrees C. A two-stranded hairpin ribozyme, divided into two fragments between G45 and U46 in a hairpin loop, showed higher ligation activity than the nondivided ribozyme. The two stranded ribozyme also catalyzed an exchange reaction of the 3'-portion of the cleavage site.
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