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  • Title: Purification and characterization of an N-acetyllactosamine-specific lectin from larvae of a moth, Phalera flavescens.
    Author: Umetsu K, Yamashita K, Suzuki J, Yamashita T, Suzuki T.
    Journal: Arch Biochem Biophys; 1993 Feb 15; 301(1):200-5. PubMed ID: 8442662.
    Abstract:
    A lectin (Phalera flavescens agglutinin, PFA) of a moth (P. flavescens) has been isolated from hemolymph by DEAE-Toyopearl followed by Cellulofine GCL-1000 chromatography. On size-exclusion chromatography, PFA showed a molecular mass of about 74 kDa, and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis it was separated into two different subunits, glycosylated 18-kDa and unglycosylated 17-kDa subunits, suggesting that PFA occurs as a heterotetrameric protein. N-Acetyllactosamine and laminin inhibited the hemagglutinating activity of PFA. Studies involving Synsorbs showed that PFA interacts with the Gal beta 1-->4GlcNAc group and that nonsubstituted hydroxyl groups at the C-2 and C-4 positions of the galactose residue were essential for interaction with PFA, whereas substitution at the C-3 position of the galactose did not inhibit the interaction between PFA and sugar chains associated with N-acetyllactosamine. PFA is not mitogenic for native or desialylated mouse thymocytes or splenocytes.
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