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  • Title: Incorporation of arachidonic acid in a human cancer gastric tumor cell line (HGT) at various stages of cell proliferation.
    Author: Denizot Y, Najid A, Rigaud M.
    Journal: Cancer Lett; 1993 Feb; 68(2-3):199-205. PubMed ID: 8443793.
    Abstract:
    Human cancer gastric cells (HGT) in culture were labeled with [14C]arachidonic acid. The kinetic of acylation of arachidonic acid was similar using cells after 3, 5 and 7 days of culture. However, the duration of the proliferation enhanced the acyltransfer to triglycerides and inhibited the transfer to phospholipids. After 8 h of incubation with [14C]arachidonic acid the radioactivity was predominantly recovered in the phosphatidylcholine and phosphatidylethanolamine species. Prelabeling-chase experiments indicated a preferential incorporation of arachidonic acid into phosphatidylcholine followed by a transfer of arachidonate to phosphatidylethanolamine.
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