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  • Title: Laboratory diagnosis of keratomycosis: comparative evaluation of direct microscopy and culture results.
    Author: Vajpayee RB, Angra SK, Sandramouli S, Honavar SG, Chhabra VK.
    Journal: Ann Ophthalmol; 1993 Feb; 25(2):68-71. PubMed ID: 8447653.
    Abstract:
    In a prospective study, we evaluated the sensitivities of direct microscopy using potassium hydroxide 10% wet-mount preparations and culture for definitive diagnosis of keratomycosis in 171 clinically suspected fungal corneal ulcers. Although cultures were positive in 88 eyes (51.46%), the fungus could be demonstrated by KOH preparation in 94.3% (83 of 88) of the culture-proved cases and 93.6% (160 of 171) of the overall eyes. Direct microscopic examination of a KOH wet-mount preparation is a rapid, reliable, and superior technique of laboratory diagnosis. It is particularly important for the early initiation of antifungal treatment in mycotic keratitis.
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