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  • Title: Enzymatic assay of fructose-1,6-diphosphate for the measurement of its utilization by tissues.
    Author: Galzigna L, Manani G, Giron GP, Burlina A.
    Journal: Int J Vitam Nutr Res; 1977; 47(1):88-91. PubMed ID: 844954.
    Abstract:
    The enzymatic analysis of fructose-1,6-diphosphate by a coupled assay utilizing triose-phosphate isomerase, glycerol-3-phosphate dehydrogenase and aldolase was carried out in rat serum and human plasma after fructose-1,6-diphosphate administration. The rate of disappearance of fructose-1,6-diphosphate was determined and compared to the activity of aspecific phosphatase. The uptake of fructose-1,6-diphosphate by rat liver and ileum was also determined with the same method.
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