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  • Title: Chromosome organization of Streptococcus mutans GS-5.
    Author: Hantman MJ, Sun S, Piggot PJ, Daneo-Moore L.
    Journal: J Gen Microbiol; 1993 Jan; 139(1):67-77. PubMed ID: 8450309.
    Abstract:
    Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).
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