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  • Title: Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa.
    Author: Suzuki A, Handa S, Ishizuka I, Yamakawa T.
    Journal: J Biochem; 1977 Jan; 81(1):127-34. PubMed ID: 845131.
    Abstract:
    Seminolipid (1-O-alkyl-2-O-acyl-beta-3'sulfogalactosyl glycerol) was detected on a micro-scale by high performance liquid chromatography (HPLC) using a UV detector. The benzoylation method of McCluer and Evans ((1973) J. Lipid Res. 14, 611-617) was used to convert seminolipid to a derivative with UV absorption. Total lipid from germinal tissue was benzoylated and the product was separated by Florisil and Sephadex LH-20 column chromatographies. The seminolipid obtained in the effluent from the Sephadex LH-20 column was determined by HPLC. Eighty percent recovery of 35S-labelled seminolipid was obtained by this method. The method was used to determine the seminolipid content of boar spermatozoa: 980 nmoles of seminolipid was found per gram of packed cells.
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