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  • Title: A 5 kDa protein (SCS23) from the 30 S subunit of the spinach chloroplast ribosome.
    Author: Wada A, Koyama K, Maki Y, Shimoi Y, Tanaka A, Tsuji H.
    Journal: FEBS Lett; 1993 Mar 15; 319(1-2):115-8. PubMed ID: 8454041.
    Abstract:
    The proteins of the 30 S ribosomal subunits from spinach chloroplasts were investigated using a radical-free and highly reducing (RFHR) method of two-dimensional polyacrylamide gel electrophoresis (PAGE). Twenty-three proteins were resolved on the gel down to the smallest protein of 5 kDa. The N-terminal amino acid sequence of the 5 kDa protein showed no homology with that of any other protein stored in databases, and the copy numbers were estimated to be 0.88 +/- 0.16 and 0.72 +/- 0.04 in the 30 S subunits and the 70 S ribosomes, respectively. The results suggest that the 5 kDa protein, which we have called SCS23, may be an essential ribosomal protein specific to spinach chloroplasts.
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