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  • Title: Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva.
    Author: Lam YW, Chan DC, Rodriguez SY, Lintakoon JH, Lam TH.
    Journal: J Chromatogr; 1993 Jan 29; 612(1):166-71. PubMed ID: 8454696.
    Abstract:
    A high-performance liquid chromatographic assay was developed to determine salivary chlorhexidine concentration. Saliva sample (200 microliters) was extracted into methylene chloride. Chromatographic separation was achieved with a C18 column using a mobile phase of acetonitrile-0.05 M sodium acetate and 0.005 M heptanesulfonic acid (40:60, v/v). The standard curve was linear from 0.051 to 20.48 micrograms/ml (r2 > or = 0.997). Intra-day and inter-day coefficients of variation were < 5.5 and < or = 9%, respectively. The assay is rapid, sensitive, simple, and successfully used for quantitating salivary chlorhexidine content released from a chlorhexidine-impregnated resin worn by patients.
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